Lab notes 9/9/08

Selective Media

  • Media that prevents some bacteria growth.

EX: Mannitol Salt Agar (only bacteria that can survive high salt can grow MSA)
Differential Media

  • Media that lets you see whether bacteria can eat different things
  • EX:DNase agar ( if bacteria can break down DNA. The agar changes from blue green to clear where the bacteria are)

Kirby-Bauer Test for Antibiotic- R
1. Suspend ~1 small colony of unknown bacteria in 1 mL of sterile broth
2. Make "lawn" of bacteria by swabbing broth and bacteria on Mueller Hinton (MH) plate
3. Place 3 antibiotic disks per plate ( have one -ctl disk)


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