Gram Staining

1. Drop of bacteria broth- let it air dry

2. Heat fixing- pass slide through flame 3-5 times

3. Crystal Violet - 1 minute

4. Rinse and Blot (with bibulous paper)

5. Gram Iodine - 1 minute

6. Rinse and Blot

7. Ethanol - 7 to 15 drops (til becomes clear)

8. Rinse and Blot

9. Saframin - 1 minute

10. Rinse and Blot

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