Gram Staining and Hand Washing Results

More Bacteria on…
-Unwashed hands: 19
-Washed hands: 9
-No difference: 8
-R hand: 20
-L hand: 12
-No diff. R vs. L: 4
-Dominant Hand: 19
-Non-dominant hand: 13
-Not applicable: 4

Gram Staining
1. Drop of bacteria
broth on slide->air dry
2. Heat Fixing
3. Crystal Violet 1min
4. Rinse and Blot
5. Gram Iodine 1min
6. Rinse and Blot
7. EtOH:7-15 drops
8. Rinse and Blot
9. Safranin 1min
10. Rinse and Blot

lab
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