Exam 3 Potential Questions

Please post your questions here for possible inclusion on Exam 3.
1. Sign each of your questions, both in the editing window (this one) and in the "short description of changes" box below. In the "short description of changes" box write "(your name) added questions 1-4" for instance.
2. Submit as many questions as you would like. For any of your questions to be considered, you must submit a minimum of three questions (although you don't have to post them all at the same time).
3. I will only accept one question per person for inclusion on exam 3. I won't necessarily use questions from everyone who submits questions.
4. I reserve the right to change the wording of questions to clarify them, but I promise not to change the wording in such a way as to change the meaning of a question that's been submitted.

Remember this test was moved from the orignial date of Wednesday December 5th to Friday November 30th
Ok, now start writing questions. The earlier you get started, the better it will work out for you.

1.) Draw a virus structure
Some of the things you might want to include are: nucleic acid, capsid, capsomere, phospholipid membrane from host cell, and glycoproteins

2) In which of the following situations is natural transformation is most likely to occur?
a. environmentally favorable conditions in which bacteria can thrive
b. high population densities of like species
c. an environment containing many diverse bacterial species
d. environmentally stressful conditions in which bacteria are dying

3.) Which of the following is NOT true of a transposon?
a. They can move around in your genome
b. They are thought to cause some illnesses/diseases
c. viral DNA is in the viral genome
d. They can sometimes disrupt coding for a certain protein and change it into a different protein

4) Which of these is part of Viral attachment/absorption
a. capsid dissolves when cell ingests foreign objects
b. virus bumps into host cell
c. 1:106 bumps sticks to cell
d. can only interact if it has the right shaped proteins
5) Put these events in order for artificial transformation and name one benefit this has for humans

_ Plate on agar with abx

_ Plasmid is cut with restriction enzyme

_ Bacteria is forced to take gene

_ If bacteria grew on plate then it must have plasmid

_ Patch of DNA is put into plasmid along with abx-gene

6) Conjugation involves
a. An F+ bacteria having a pilin gene on its plasmid
b. The plasmid is copied and given to the other bacterium by the pilin
c. An F- bacteria does not have the pilin
d. The F+ attaches to the F- with its pilin
***Just a note on this one, I thought that conjugation is not just between 2 gram negative bacteria that do and do not have pilins. It's also between 2 related bacteria, one of which contains a plasmid and one does not. It's the genetic exchange of that plasmid. So the question as is should say just for gram negative bacteria with pilins. -Alicia

7) Which of the following are TRUE about autoinducers (AIs)?
a. AIs are continually being released by bacteria that are quorum sensed
b. Homoserine lactone is the AI used by gram + and gram - bacteria
c. only at high concentrations of AIs a change in gene expression occurs
d. AIs bind to a genes promoter sequence and inhibits the transcription of the AI gene
8) Put the steps of spore formation in the correct order
__ dipicolinate layer covers peptidoglycan layer
__ asymmetric cell division - forespore created and axial filament enters forespore
__ bacterial chromosome detaches from cell envelope and moves to center of bacterium
__ peptidoglycan covers forespore
__ mother cell degraded by environment freeing spore
__ mother cell engulfs forespore

9) What answer is not true about B.Subtilis?
__ species can be transformed
__ individual bacteria in population become comptent by expressing com K protein
__ expression on com K protein is deliberate across genetically identical individuals in a protein
10) What is the difference between virulent and temperate phage lifecycles?
__ virulent phages integrate into host DNA
__ Virulent phages run copies and kills host
__ Virulent and temperate phages both kill off host cell after intergrating into host DNA
__ temperate phages only finish the lifecycle if damage to DNA occurs
11) When is quorum sensing most likely to occur?
__ High concentration of Autoinducers
__ Low concentration of Autoinducers
__ In gram negative bacteria
__ In gram positive bacteria

12) Which of the following contribute to Black Plague infection?
a. Yersinia pestis
b. Rats biting humans
c. Blood clots in the guts of fleas
d. swollen lymph nodes with lots of bacteria immune cells called "booboos"
e. Hemagglutinin
f. Plasminogen activator
g. Flesh eating bacteria
h. Strep throat

13) Last September, a 7 year old was having symptoms that included a severe and sudden sore throat, a fever, swollen lymph nodes, and a bright red throat or dark red spots on the roof of the mouth at the back near the throat. Later puss pockets formed on the back of the child's throat. What sickness that we discussed in class do you think this child has? Explain your reasoning.

14) Explain why after opening Aunt Maizie's canned green beans you should reheat the contents. If the contents were not reheated what would you be at risk of exposure to? Explain your reasoning.

15. Which of the following are TRUE of toxigenic disease?
a. Symptoms caused by microbe invading host cell's tissues
b. Bacteria causing disease live in non-sterile sites
c. Host cells are disabled or damaged by exotoxins secreted by the microbe
d. Examples include botulism and cholera
Ashley S

16. Differentiate between a vector and a reservoir.

Ashley S

17. Compare and contrast infectious and contagious.
a. disease where symptoms in the host are caused by replication of the microbe.
b. contagious can be transmitted from host to host.
c. symptoms caused by microbe invasion of a host tissue.
d. where microbe that is a disease that exists in nature.

18. Virulence factors:
a. measure of the damage by a microbe to a host.
b. any chemical that contributes to microbes ability to cause symptoms in a host.
c. protein secreted by growing bacteria that disables or damages host cell.
d. symptoms caused by microbe invasion of host.

19. Describe Botulism. What shouldn't babies eat that has the botulism toxin SPORES in it? What will it do to them?


20. Describe Cholera.


21. There are three (3) virus shapes. Name the three and give a single example of each of them.

Ben Pottebaum

22. Replication of nucleic acids have three (3) different types. They all deal with viral replication. Name the three different types of viruses and give a single example of each one.

Ben Pottebaum

23. There are consequences of viral infection on host cell. List the three (3) consequences and elaborate on each of them to give a better understanding of each.

Ben Pottebaum

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